Table 2.

A Comparison of the Three Bleach Correction Strategies Used in this Study to Correct for the Continuous Bleaching in FRAP

Correction Strategy	Assumptions	Requirements on data	Suitable experimental systems
External Exponential	Complete recovery of fluorescence	FRAP recovery recorded until a high degree of convergence	In vitro molecular structures (e.g., SLBs)
Internal Exponential	Monoexponential bleaching	FRAP recovery recorded until a very high degree of convergence needed for reliable fitting with Eq. 3	In vitro molecular structures (e.g., SLBs)
Reference Area	The FRAP region and the reference area have almost identical bleaching profile	Intensity trace from a reference area (optimally recorded simultaneously with the FRAP recovery)	Cells and in vitro systems