Table 3.

Average D_FCS and D_FRAP Values for Simulations and Measurements in SLBs and Live Cells for Different Bleach Correction Strategies in FRAP

Correction Strategy	DFCS/μm2 s−1	DFRAP/μm2 s−1
(1)	(2)	(3)
Simulation (0.2 μm2 s−1)	0.20 ± 0.01	0.19 ± 0.05	0.09 ± 0.08	0.22 ± 0.03
Simulation (0.5 μm2 s−1)	0.49 ± 0.02	0.74 ± 0.34	0.49 ± 0.02	0.59 ± 0.18
Simulation (1.0 μm2 s−1)	1.00 ± 0.01	1.24 ± 0.41	0.98 ± 0.03	1.20 ± 0.35
DOPC SLB	2.6 ± 0.2	4.0 ± 0.3	3.9 ± 0.5	7.2 ± 1.0
GFP-GPI in CHO	0.33 ± 0.10	—	—	0.24 ± 0.16

(1) External and (2) internal exponential correction and (3) reference area correction.