Figure 6.

(a) Time-lapse images (i–iii) of the effect of cycle frequency and drug administration on cell force. (iv) Increasing cycle frequency decreases cell force (1× = 7% force reduction, 4× = 37%; continuous = 41%). Similarly, increasing drug concentration while cycling at 1× also decreases force (10 μM = 38%; 100 μM = 57%). Despite force magnitude reduction being similar, ROCK inhibition does not appear to reach steady state within 30 min. Error bars represent standard error (n = 6 for each case). (b) Reduction of cancer cell blebbing through probe-induced stretch, as shown by (i–iii) time-lapse images and (iv) force evolution upon onset of transition from blebbing to lamellipodial behavior (n = 11). To see this figure in color, go online.