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. 2016 Jul 15;10:178. doi: 10.3389/fncel.2016.00178

Figure 4.

Figure 4

Loss of olfactory input drives changes in microglial morphology. (A,B) (i) More ramified microglial morphology in open OB vs. (ii) more amoeboid microglial morphology in occluded OB. (A) Scale bars: 50 μm. (B) Lower panels show reconstructions (red dots: somata). Scale bars: 20 μm. (C) Shorter process length in occluded OBs (*p = 0.031, t = 2.82, t-test). (D) Fewer process branch points in occluded OBs (*p = 0.029, t = 2.85, t-test). (E) Lower process complexity in occluded OBs [effect of occlusion, p < 0.001, F(1, 120) = 72.31; *p < 0.05; **p < 0.01 at particular radii from soma; two-way repeated measures ANOVA with Sidak's test]. (C–E): n = 4 pairs of open and occluded OBs from 4 naris-occluded mice. All comparisons are of mean values per OB. A total of 54 microglia in occluded OBs and 52 microglia in open OBs were reconstructed (7–18 microglia per OB).