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. 2016 Jul 15;7:1028. doi: 10.3389/fpls.2016.01028

FIGURE 1.

FIGURE 1

Schematic comparison of v-ATPase A from targeted insect pest, Diabrotica virgifera and non-target Sinella curviseta. (A) Schematic drawing of the primary structures of v-ATPase A cDNA from S. curviseta and D. v. virgifera. (B) The alignment of a highly conserved region within the ORFs of v-ATPase A from S. curviseta and D. v. virgifera. This 400 bp fragment, which has the highest sequence similarity among all the tested surrogate species, was selected as the target template to synthesis insecticidal dsRNAs. (C) This schematic drawing shows the distribution and number of identical sequences, ranging from 19 to 23-mer matches, imbedded in the ORFs of v-ATPase A from S. curviseta and D. v. virgifera.