Table 1.
Primers used in this study.
| Primers name | Sequence (5′–3′)∗ |
|---|---|
| dsRNA synthesis | |
| dsSC F | TAATACGACTCACTATAGGGAGAACACTATTTCCATGTGTTCAG |
| dsSC R | TAATACGACTCACTATAGGGAGAGCATCTCAGCCAATCG |
| dsDVV F | TAATACGACTCACTATAGGGAGAGCTCTTTTCCCATGTGTAC |
| dsDVV R | TAATACGACTCACTATAGGGAGAGCATTTCAGCCAAACG |
| dsGUS F | TAATACGACTCACTATAGGGAGAGGGCGAACAGTTCCTGATTA |
| dsGUS R | TAATACGACTCACTATAGGGAGAGGCACAGCACATCAAAGAGA |
| RT-qPCR | |
| v-ATPase A RT-qPCR F | TGTCTGGATCTGCTATG |
| v-ATPase A RT-qPCR F | CTTGGTGCGTGATAAAG |
| 28S RT-qPCR F | CACGAGTCAGTCGATCCTAAAC |
| 28S RT-qPCR R | ACCAGATTCCCTTTCACCTTATC |
| Gene cloning | |
| Degenerate primer F | AGATGTCCGGATCNGCTATGTACGA |
| Degenerate primer R | ACGAGCAGCCACAGGCATGTT |
| 5′ RACE | |
| Universal Primer A Mix | CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT |
| v-ATPase A R | GACGGTCTTGTAGAAGGGACAGAA |
| 3′ RACE | |
| Universal Primer A Mix | CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT |
| v-ATPase A 1F | GGCCAAATCAATTTACATTCC |
| v-ATPase A 2F | ATATGTCGCTGAAGCTGGAAGTTAC |
| Full sequence verification | |
| Primer F | GTGCTCGGTTGAAGTGGGATTGAA |
| Primer R | ATGTACGAGTTGGTTCGTGTCGGT |
∗T7 = TAATACGACTCACTATAGGG.