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. 2016 Jul 13;36(28):7535–7545. doi: 10.1523/JNEUROSCI.3358-15.2016

Figure 1.

Figure 1.

Retrograde label of feedback to the MOB (MOB). A, Schematic outlining experimental design. B1, Neurons labeled with mCherry rabies (red) and DAPI nuclear counterstain (blue). B2, 3D reconstruction of injection site in the olfactory bulb. Gray indicates the tissue edge; blue, GCL; red, mCherry-positive neurons. Scale bar, 500 μm. C, Variability in injection size across experiments (C, insert) and variability in injection location; each color corresponds to an experiment. Sphere center is the location of the injection center in the GCL. The sphere radius is the injection size. Scale bar, 1 A.U. D, AON with areas of retrogradely labeled neurons in red. d, Dorsal; l, lateral; lot, lateral olfactory tract; L1, layer 1 of AON; pv, posteroventral; m, medial; aoc, anterior commissure. Retrogradely labeled AON neurons from ipsilateral. Scale bar, 500 μm. E1, E2, AON (E1) and contralateral AON (E2) from D. Scale bar, 25 μm. F, Piriform cortex (blue) with retrogradely labeled neurons in red. L1, Layer 1; L2, layer 2; L3, layer 3; Pir, piriform cortex. Scale bar, 500 μm. G, Morphology of layer 2 piriform neurons. Scale bar, 50 μm.