Figure 10. Cardiac application of CLARITY .

Representative images of whole mouse hearts through the CLARITY clearing process. A, hearts are perfused with a hydrogel monomer solution and polymerized to create a hydrogel–tissue hybrid. B, excess gel is removed as hearts are prepped for electrophoretic tissue clearing (ETC) treatment. C, tissues become fully transparent after the extraction of unbound lipid membranes. D, montage of high resolution light sheet images from human LV highlighting fibre architecture and trabeculation. Autofluorescence is sufficient for imaging.