Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jun 27;5(6):e236. doi: 10.1038/oncsis.2016.45

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 Macmillan Publishers Limited

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Deletion of Ric-8A in murine melanocytes confers a modest cell proliferation advantage, but does not confer TPA- and/or CTX-independent growth. (a) Bright-field images of untreated or 4OHT-treated immortalized Ric-8A^Flox/Flox; Rosa-CreER^+/⁻ melanocytes grown in the presence of CTX, or TPA, or both for 4 days. (b) Quantitative western blot analyses of Ric-8A, Gαi_1/2, Gαq/11, Gα13, Gαs, Gαo and Gβ₁₋₄ levels in Cre or GFP (control) lentivirus, or 4OHT-treated and untreated cultured melanocyte cell lines. Relative GAPDH or α-tubulin levels are shown as normalization controls. (c) Cell proliferation analyses of untransduced, and Cre or GFP (control) lentivirus-infected Ric-8A^Flox/Flox; Rosa-CreER^+/− melanocytes. Error bars are the mean±s.e.m. of experiments performed in triplicate.