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. 2016 May 30;5(5):e229. doi: 10.1038/oncsis.2016.22

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Copyright © 2016 Macmillan Publishers Limited

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Expression of ANCCA/PRO2000 in liver cell lines. (a) Western blotting analysis of ANCCA/PRO2000 protein expression in liver cell lines L02, HepG2, Hep3B, Huh7 and Hep40. (b) qRT–PCR and western blotting analyses confirmed that downregulaiton of ANCCA/PRO2000 in HepG2 and Huh7 via siANCCA (100 pmol) and upregulation in L02 cells by pcDNA3.1-ANCCA (2 μg). (c) qRT–PCR and western blotting analyses of ANCCA/PRO2000 expression in HepG2 and Huh7 cells infected with lentiviral particals of shANCCA. Error bars indicate mean±s.d. from three independent experiments. *P<0.05; **P<0.01. P-values were determined using independent samples T-test.