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. 2016 May 30;5(5):e229. doi: 10.1038/oncsis.2016.22

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Copyright © 2016 Macmillan Publishers Limited

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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ANCCA/PRO2000 promoted the cell migration and invasiveness of HCC cells in vitro. (a) Cell migration was quantified by wound-healing assay. Cells were imaged immediately (0 h) and 48 h after scratches were created to measure the percentage of wound-healed area. Representative images at different time points are shown. Scale bar, 150 μm. (b, c) Transwell cell migration assay (b) and Matrigel invasion assay (c). Representative photographs are showed in the upper panels. Quantitative measurements are showed in the lower panels. Data represent mean±s.d. of three independent experiments. Scale bars, 100 μm. *P<0.05; **P<0.01.