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. 2016 May 30;5(5):e229. doi: 10.1038/oncsis.2016.22

Figure 7.

Figure 7

miR-520a is an intermediate regulator between ANCCA/PRO2000 and E2F2. (a) ANCCA/PRO2000 inversely regulates miR-520a expression in HepG2 and Huh7 cells. (b) qRT–PCR analysis of miR-520a expression in tumor xenografts. (c) Predicted binding sites of miR-520a in E2F2 3′-UTR. (d) E2F2 expression was analyzed by western blotting in HepG2 and Huh7 cells after transfection with miR-520a mimics alone, the combination of miR-520a mimics and pcDNA3.1-E2F2, or the corresponding controls. (e) Cell growth curves of HepG2 and Huh7 cells. (f) Plate clone-forming assay of HepG2 and Huh7 cells. Representative photographs are shown, and the numbers of colonies were counted. (g) FACS analysis of distribution of cells in each cell cycle phase. Data are shown as mean±s.d. derived from three independent experiments. *P<0.05; **P<0.01, versus siCtrl, mimics control or miR-520a mimics+pcDNA3.1.