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. 2016 May 30;5(5):e226. doi: 10.1038/oncsis.2016.32

Figure 7.

Figure 7

ROR1 and ROR2 overexpression in the A2780 parental cell line. (a) ROR1 is overexpressed at the mRNA level following ROR1 plasmid transfection. No effect seen on ROR2 levels. qRT–PCR was performed in triplicate and normalised to three different housekeeping genes (SDHA, HSPCB and RPL13A). Results represent an average of three experiments. Error bars represent the s.d. of the mean. (b) ROR2 is overexpressed at the mRNA level following ROR2 plasmid transfection. No effect seen on ROR1 levels. qRT–PCR was performed in triplicate and normalised to three different housekeeping genes (SDHA, HSPCB and RPL13A). Results represent an average of three experiments. Error bars represent the s.d. of the mean. (c) Simultaneous transfection of ROR1 and ROR2 plasmids in A2780 increases mRNA levels of ROR1 and ROR2. qRT–PCR was performed in triplicate and normalised to three different housekeeping genes (SDHA, HSPCB and RPL13A). Results represent an average of three experiments. Error bars represent the s.d. of the mean.