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. 2016 May 9;5(5):e222. doi: 10.1038/oncsis.2016.33

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Copyright © 2016 Macmillan Publishers Limited

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Screening for apoptotic regulators identifies BIRC5 as an important survival gene in chondrosarcoma cell line JJ012. (a) Occupied area of cells present in the well as a percentage to control siRNA of 51 apoptosis-related genes. Mean values and range of duplicates are shown. (b) Raw data measured as Hoechst area of controls used in the siRNA screen. Dots indicate individual measurements and mean values are shown for each control. siCTR indicates control siRNA of either GFP or GAPDH. (c) Area of cells as a percentage to control siRNA of selected hits. BIRC5 shows 4/4 siRNAs that mimic the smart pool. Data represent means of duplicate values with range. Black bars represent the smart pool and gray bars represent individual siRNAs.