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. 2016 May 9;5(5):e224. doi: 10.1038/oncsis.2016.35

Figure 2.

Figure 2

Overexpressed miR-186 inhibited cell proliferation, glucose uptake, lactate accumulation, ATP/ADP ratio, increased apoptosis and NAD+/NADH ratio in MKN45 and SGC7901 cells. (a) MiR-186 expression levels were evaluated using qRT–PCR in agomir-186- and antagomir-186-transfected MKN45 and SGC7901 cells. (b) CCK8 assays were performed to determine the cell viability of MKN45 and SGC7901 cell lines stably overexpressing or knockdown of miR-186. (c) The apoptotic percentages of MKN45 and SGC7901 cells stably overexpressing miR-186 by flow cytometry. LR, early apoptotic cells; UL, necrotic cells; UR, terminal apoptotic cells. (d) The glucose uptake assay, (e) lactate accumulation, (f) ATP/ADP ratio and (g) NAD+/NADH ratio of MKN45 and SGC7901 cell lines stably overexpressing or knockdown of miR-186. Data were presented as mean±s.d. from three independent experiments. *P<0.05, **P<0.01.