DGAT1 inhibits PA-induced osteoclastogenesis. (A) RT-PCR analysis for DGAT1 mRNA; ***p < 0.001, n = 6. (B, C) Neutral lipid staining with Oil-Red-O (B) and GFP fluorescence (C) in RAW 264.7 cells after 5 days of treatment with PA and Ad-GFP, or Ad-DGAT1 (MOI: 250). (D) RT-PCR analysis for TRAP and TNFα mRNAs; *p < 0.05, ***p < 0.001, n = 4 to 6.