Figure 3.

HFD suppresses TRPV1 channel activity in epididymal and subcutaneous preadipocytes, and dietary capsaicin antagonizes this. Representative traces of time courses of capsaicin‐stimulated (1 μM) Ca^{2 +} influx measured in Fura‐2 AM loaded primary epididymal and subcutaneous preadipocytes obtained from NCD‐fed, HFD‐fed or HFD + CAP‐fed WT [EF: A–C; SCF: G–I] TRPV1^−/− [EF: D and E; SCF: J–L]. (F) The average change in fluorescence ratio after the addition of 1 μM CAP ± SEM is given for epididymal and subcutaneous preadipocytes of WT and TRPV1^−/− mice (n = 40) under each condition and used for the imaging experiments. ** P < 0.05; significantly different as indicated.