Figure 8.

Dietary capsaicin activates SIRT‐1 via TRPV1‐CaMKIIα‐AMPK‐dependent mechanism. (A) and (B) Representative Western blots for the expression levels of CaMKIIα/p‐CaMKIIα (Thr²⁸⁶) and AMPKα/p‐AMPKα (Thr¹⁷²) in 40 μg of SCF lysate (β‐actin, loading control; n = 40). (C) and (D) Representative Western blot shows immunoprecipitation of SIRT‐1 (10% input) and immunoblotting with anti‐SIRT‐1 antibody. (D) Representative blot showing SIRT‐1 phosphorylation using phosphoserine and threonine antibodies in the immunoprecipitated samples.