Figure 2.

Leptin did not induce HSP90 expression. HEK 293‐Ob‐Rb cells were stimulated with leptin (0.5 μg·mL⁻¹) for 0.25–10 h. (A) Western blotting was performed using specific antibodies for HSP90, GAPDH, phospho‐STAT3 (Tyr⁷⁰⁵) and STAT3. (B) A densitometoric analysis of phospho‐STAT3 and HSP90 was conducted using image j analysis software. Each set of data was expressed as fold increase over control cells. n = 5 for phospho‐STAT3 and HSP90 experiments.