Figure 5.

An HSP90 inhibitor inhibited leptin‐induced increases in nuclear phospho‐STAT3 staining. Fluorescence images of phospho‐STAT3 staining in SH‐SY5Y‐Ob‐Rb cells. Cells were treated with geldanamycin (GA: 10 μM) for 1 h and then stimulated with leptin (0.5 μg·mL⁻¹) for 15 min. Left panels show PI nucleus staining (red). Second panels show phospho‐STAT3 staining (green). Third panels show merged photo of PI and phospho‐STAT3 staining. Geldanamycin inhibited leptin‐induced increases in nuclear phospho‐STAT3 staining. PI, propidium iodide. Original magnification: ×63.