Table 2.
Flow cytometry analysis of lymphocytes from rat kidneys on day 7 post‐IRI
| Parameters | Control (n = 3) | VDD (n = 3) | IRI (n = 7) | VDD+IRI (n = 7) |
|---|---|---|---|---|
| CD4+ | 2371 ± 457 | 2281 ± 1050 | 2175 ± 469 | 4432 ± 746i |
| CD8+ | 1399 ± 439 | 2016 ± 509 | 1255 ± 317 | 3451 ± 485i |
| CD4+/IL17+ | ND | ND | 424 ± 188 | 2055 ± 584i |
| CD4+/IFN‐γ + | ND | ND | 40 ± 21 | 237 ± 52i |
| Treg | 34 ± 8.4 | 26 ± 2.8 | 12 ± 3.1 | 9.6 ± 2.4i |
| Th17/Treg | ND | ND | 1.8 ± 0.5 | 27 ± 7i |
| Th1/Treg | ND | ND | 20.5 ± 5.2 | 265.1 ± 6.9i |
Control, sham‐operated (standard diet); VDD, vitamin D deficient (vitamin D‐free diet 35 days, sham surgery at 28 days); IRI, ischemia/reperfusion injury (standard diet 35 days subjected to bilateral renal ischemia for 45 min on day 28); VDD+IRI, vitamin D deficient+ischemia/reperfusion injury (vitamin D‐free diet 35 days and subjected to bilateral renal ischemia for 45 min on day 28); CD4+, CD8+, IFN‐γ +, and Th17 cells defined as described previously (Mehrotra et al. 2015), T‐regulatory cells defined as % of Foxp3+ cells. Data are mean ± SEM. i P < 0.05 IRI versus VDD+IRI.