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. 2016 Jul 8;4(13):e12858. doi: 10.14814/phy2.12858

Figure 5.

Figure 5

14‐3‐3ζ silencing inhibits cell migration after wounding in different lines of IECs. (A) Representative immunoblots of 14‐3‐3ζ and HuR. IEC‐6 cells were transfected with control siRNA (C‐siRNA) or si14‐3‐3ζ, and whole‐cell lysates were harvested 48 h thereafter. (B) Images of cell migration after wounding in IEC‐6 cells: (a) 0 h after wounding, (b) 6 h after wounding, (c) 6 h after wounding in cells transfected with C‐siRNA, and (d) 6 h after wounding in cells transfected with si14‐3‐3ζ. (C) Summarized data of cell migration 6 h after removal of part of the monolayer in (a) IEC‐6 cells, (b) IEC‐Cdx2L1 cells, and (c) Caco‐2 cells transfected with C‐siRNA or si14‐3‐3ζ for 48 h followed by cell migration assays. Data are means ± SEM from six dishes. *P < 0.05 compared with cells transfected with C‐siRNA. IECs, intestinal epithelial cells.