Fig. 2.

U937VbR and MES-SA/Dx5 overexpress ABCB1 mRNA and its protein product P-gp. (A) Real-time quantitative PCR (RT-qPCR) of the ABCB1 mRNA transcript level in MES-SA, MES-SA/Dx5, U937 and U937VbR cell lines. Raw expression values were normalized to the internal reference gene, β-actin and expressed as a fold increase to respective cells lines. (B) Fold-increase in P-gp. Immunofluorescent staining of P-gp on the surface of parental and resistant cell lines as determined by flow cytometry. Values are the mean (± SD) of triplicates.