Figure 6. Unveiling possibly mossing regulatory interactions.

Exploration of likely missing allosteric interactions in the methionine cycle. Positive and negative interactions are represented by green and red arrows respectively, whereas missing interactions are represent by grey arrows. Two sequential additions were performed but only the positive activation of MATIII by AdoMet was recovered from the incomplete model (p-value = 3.2·10⁻⁴ < 10⁻³). Addition of the negative inhibition of GNMT by AdoHcy was not found to be a significant interaction despite its presence in the original model (Bayes factors <3.0). This could point to the presence of compensatory mechanisms in the network (see Supplementary Fig. S6).