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. 2016 Jul 15;6:29663. doi: 10.1038/srep29663

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Copyright © 2016, Macmillan Publishers Limited

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(A) Representation of Wilby’s scale of toxicity testing following CuO NR exposure. The complete description of the structural changes associated with the numerical score is provided in the Table S1. (B) Median score of polyps exposed to increasing concentrations of CuO NR. Median score was calculated for each concentration and time point on groups of 25 animals. Significance between the control and the treatment groups were performed by adopting two way ANOVA with Dunnet’s multiple comparison test (****p < 0.001)). (C) The intracellular Cu content was evaluated by elemental analysis. Polyps were exposed to CuO NR for 24 h. The Cu content is represented in ppm.