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. 2016 Jul 12;7:12135. doi: 10.1038/ncomms12135

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(a) Schematic diagram of various truncated or mutant derivatives of Claspin generated. BP1 and BP2: basic patch1 and 2. CKBD: Chk1 binding domain. (b) Mutant Claspin polypeptides tagged with 3x Flag at the C termini were expressed in 293T cells and pulled down with M2 Flag beads (Flag IP). Co-pulled down proteins were analysed by western blotting to detect the proteins indicated. Asterisk, IgG; black arrowheads, full-length or mutant Claspin polypeptides pulled down by Flag antibody. (c) Loss of interaction with Cdc7 in Cdel1 and Cdel6 mutants, whose structures are shown in the upper drawing. Interaction was examined as in b.