Table 1. Experimental factors predicting α-diversity of leaf- and root- associated bacterial communities.
|
Leaf |
Root |
|||
|---|---|---|---|---|
| Shannon | Chao1 | Shannon | Chao1 | |
| R2 | 0.56 | 0.63 | 0.68 | 0.72 |
| Site | F 2,48=44.1 | F 2,49=51 | F 2,41=140 | F 2,34=141 |
| P=1.4e−11 | P=2.3e−12 | P<3e−16 | P<3e−16 | |
| Geno. | F4,263=1.62 | F 4,258=4.68 | F 4,28=0.648 | F 4,261=0.344 |
| P=0.17 | P=0.0023 | P=1 | P=1 | |
| Geno. × Site | F8,261=2.33 | F 8,256=0.903 | F 8,260=1.73 | F 8,259=0.94 |
| P=0.04 | P=0.51 | P=0.18 | P=0.48 | |
| Age | F 2,61=0.652 | F 2,64=1.11 | F 2,67=2.91 | F 2,57=6.08 |
| P=0.67 | P=0.67 | P=0.062 | P=0.0081 | |
| Age × Site | F 4,59=1.63 | F 4,63=1.48 | F 4,65=0.724 | F 4,56=7.85 |
| P=0.36 | P=0.36 | P=0.58 | P=8.6e−05 | |
| Year | F 1,73=7.36 | F 1,66=8.37 | F 1,54=6.73 | F 1,72=12.3 |
| P=0.01 | P=0.01 | P=0.012 | P=0.0016 | |
| Year × Site | F 2,79=0.741 | F 2,71=0.193 | F 2,88=0.153 | F 2,73=5.94 |
| P=0.96 | P=0.96 | P=0.86 | P=0.0082 | |
| Block | χ21=1.48 | χ21=6.27 | χ21=1.13 | χ21=2.08 |
| P=0.22 | P=0.025 | P=0.3 | P=0.3 | |
| Line | χ21=7.96e-13 | χ21=0 | χ21=0.0128 | χ21=0 |
| P=1 | P=1 | P=1 | P=1 | |
| Obs | F 1,273=3.08 | F 1,267=132 | F 1,180=0.4 | F 1,137=104 |
| P=0.081 | P<3e−16 | P=0.53 | P<3e−16 | |
| MiSeq run | χ21=0.754 | χ21=0.649 | χ21=1.79 | χ21=0.123 |
| P=0.77 | P=0.77 | P=0.36 | P=0.73 | |
ANOVA, analysis of variance.
Statistics describe linear random-intercept models of Shannon diversity and Chao1 richness in leaves and roots. ‘Obs', total number of observations (log-transformed). All P values were corrected for multiple comparisons using the sequential Bonferroni correction. Significance was assessed using Type III ANOVA with F tests for fixed effects and likelihood ratio tests for random effects. Bold values indicate statistically significant results after correction for multiple comparisons, P<0.05.