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. Author manuscript; available in PMC: 2017 Aug 1.

Published in final edited form as: Prostate. 2016 Apr 4;76(11):964–976. doi: 10.1002/pros.23185

Assessment of prostate volume by MRI, histology and proliferation in C57BL/6N and B6.V-Lepob/J mice at 14 and 28 weeks. A, representative T1-weighted axial 2D gradient images of the mouse prostates a-c in C57BL/6N mice and b-d in B6.V-Lepob/J mice at 14 and 28 weeks. B, prostate volume quantification. C-a, representative image of H&E staining of the prostate gland at 28 weeks. C-b, representative image of IHC staining of PCNA in the prostate at 28 weeks. A significant increase in the prostate volume was noted in B6.V-Lepob/J mice. Prostate of B6.V-Lepob/J mouse, as compared to that of a C57BL/6N mouse, shows pronounced glandular cell enlargement, markedly crowded and proliferative, virtually filled the central lumen of the gland, resulting in overall enlargement of the gland. Increase nuclear PCNA staining is noted in the prostate of B6.V-Lepob/J, compared to C57BL/6N mice. Values represent Mean ± SEM, *p<0.05, **p<0.001, compared to C57BL/6 mice. Representative H&E and IHC staining photomicrograph of the mouse prostate (×100, and ×400 magnification). Details are described in ‘materials and methods’ section.

Assessment of prostate volume by MRI, histology and proliferation in C57BL/6N and B6.V-Lepob/J mice at 14 and 28 weeks. A, representative T1-weighted axial 2D gradient images of the mouse prostates a-c in C57BL/6N mice and b-d in B6.V-Lepob/J mice at 14 and 28 weeks. B, prostate volume quantification. C-a, representative image of H&E staining of the prostate gland at 28 weeks. C-b, representative image of IHC staining of PCNA in the prostate at 28 weeks. A significant increase in the prostate volume was noted in B6.V-Lepob/J mice. Prostate of B6.V-Lepob/J mouse, as compared to that of a C57BL/6N mouse, shows pronounced glandular cell enlargement, markedly crowded and proliferative, virtually filled the central lumen of the gland, resulting in overall enlargement of the gland. Increase nuclear PCNA staining is noted in the prostate of B6.V-Lepob/J, compared to C57BL/6N mice. Values represent Mean ± SEM, *p<0.05, **p<0.001, compared to C57BL/6 mice. Representative H&E and IHC staining photomicrograph of the mouse prostate (×100, and ×400 magnification). Details are described in ‘materials and methods’ section.