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. 2016 Mar 9;41(9):2206–2214. doi: 10.1038/npp.2016.24

Figure 1.

Figure 1

Perineuronal nets (PNNs) are present around parvalbumin (PV) and pyramidal neurons in human dorsolateral prefrontal cortex (DLPFC). Images of NeuN (1—left column), PV (2—middle left column), aggrecan (3—middle right column) and Wisteria floribunda agglutinin (WFA) labeling (4—right column) in DLPFC area 9. (a) Representative images (using a x 4 objective) showing the high density of PV neurons and aggrecan- and WFA-labeled PNNs in layer 3. Numbers indicate the locations of the cortical layers. Scale bar=300 μm. (b) Low magnification images (using a × 20 objective) in layer 3 showing pyramidal cells with PNNs (arrowheads), PV neurons with PNNs (arrows), and PV neurons lacking PNNs (notched arrowheads). Scale bar=30 μm. (c) High magnification images (using a × 60 objective) showing that most PV neurons are ensheathed by PNNs (arrows) detected with both aggrecan and WFA labeling and that some PV neurons (notched arrowheads) lack PNNs labeled with either aggrecan or WFA. Scale bar=10 μm. WM, white matter.