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. 2016 Mar 16;41(9):2309–2323. doi: 10.1038/npp.2016.33

Figure 8.

Figure 8

Repeated stress impairs in vivo GABAergic regulation of lateral nucleus (LAT) neurons in adolescent rats. To test whether differences of GABA underlie effects of stress on in vivo LAT neuron firing activity, picrotoxin (PTX) was infused into the LAT during electrophysiological recordings. (a) In adult rats, PTX infusion caused an increase of LAT neuron firing (top, representative extracellular recording trace of the same neuron before and after PTX). This was observed in control (left) and stress (right) conditions (*p<0.05, Holm–Sidak's multiple comparisons test after significance in a two-way repeated-measures analysis of variance (ANOVA). (b) In adolescent rats, PTX infusion did not significantly increase the firing rate of LAT neurons (top, representative extracellular recording trace of the same neuron before and after PTX; no significance in two-way repeated-measures ANOVA). (c) In adult rats, the number of active LAT neurons encountered per electrode track was not modified by PTX in control (left) or stress (right) conditions (no significance in two-way ANOVA). (d) In adolescent rats, PTX increased the number of active LAT neurons encountered in control conditions (left; *p<0.05, Holm–Sidak's multiple comparisons test after significance in a two-way ANOVA) but not in stress conditions (right, p>0.05, Holm–Sidak's multiple comparisons test after significance in a two-way ANOVA).