Figure 1.

5-HTP SR elevates 5-HT_Ext beyond the effect of SSRI treatment alone. (a) Treatment schematic for (b–e). Mice were treated for 14 days with fluoxetine (20 mg/kg/day). Here after, minipumps delivering 5-HTP SR (100 mg/kg/day) or vehicle were implanted. All assessments were carried out 5 days (120 h) after minipump implantation. (b) Frontal cortex 5-HT_Ext (N=5–25) in absolute values, (c) frontal cortex 5-HT_Ext (N=5–25) percentage of values normalized to untreated controls, (d) frontal cortex 5-HT_Tissue (N=4–6), and (e) 5-HTP_Plasma (N=4-5) following treatment with fluoxetine, 5-HTP SR via minipumps, or the combination. (f) Effect of 5-HTP SR via minipumps (100 mg/kg/day, 5 days) or the drinking water (300 mg/kg/day) on frontal cortex 5-HT_Tissue (N=4–13) in mice not treated with fluoxetine. ZT=zeitgeber time, ie, hours after lights on on a 12-h : 12-h light–dark cycle. Data represent means±SEM. WT and 5-HT_Hypo mice were analyzed separately. (b–e) Two-way ANOVA followed by Bonferroni's post-hoc test. (f) Student's t-test. *p<0.05. Additional statistical information is available in Supplementary Table S1.