Figure 5.

Subtoxic 5-HTP IR is inefficient in elevating 5-HT_Ext beyond the SSRI effect. (a, b) Effect of adjunct 5-HTP IR on 5-HT_Ext in SSRI-treated mice. Subtoxic 5-HTP IR (3.125 mg/kg) only transiently elevated 5-HT_Ext upon dosing and only in 5-HT_Hypo mice. (N=3–4). Dotted lines represent the levels observed after adjunct 5-HTP SR (100 mg/kg/day). As in Figure 4a, 5-HTP was injected twice daily over 5 days. 5-HT_Ext was assessed at the first daily 5-HTP injection on the fifth day, 120 h after the initial injection. (c, d) Effect of adjunct 5-HTP IR on 5-HT_Tissue assessed 8 h postinjection after the last AM injection after 5 days of twice-daily treatment in mice not treated with fluoxetine. (N=3–4). Data represent means±SEM. WT and 5-HT_Hypo mice were analyzed separately. (a, b) Two-way repeated-measures ANOVA followed by Bonferroni's post-hoc test. (c, d) Student's t-test. *p<0.05. Additional statistical information is available in Supplementary Table S1.