Nucleus accumbens, insular, and prelimbic cortex, but not barrel field cortex, are affected by high dose 3,4-methylenedioxypyrovalerone (MDPV). Open bars show data for saline group and closed bars MDPV (3 mg/kg). Assessments are made between each seed region and striatal, forebrain, hypothalamic, and cortical areas. (a) Nucleus accumbens core is seed. (b) Prelimbic area is seed. (c) Insular cortex is seed. (d) Barrel field cortex is seed. *Significant differences at p<0.05 using a t-test. Data are shown as mean±SE. ACg, anterior cingulate cortex; BNST, bed nucleus of stria terminalis; D/M/L/V, dorsal/medial/lateral/ventral; IL, infralimbic; Ins, insular cortex; LOrbCtx, lateral orbital cortex; LPOA, lateral preoptic area; MPOA, medial preoptic area; NAc, nucleus accumbens; SSC FL, somatosensory cortex-forelimb area; VOrbCtx, ventral orbital cortex; VP, ventral pallidum.