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. 2016 Apr 19;2(4):e00096. doi: 10.1016/j.heliyon.2016.e00096

Fig. 6.

Fig. 6

KUS reduces ER stress and prevents apoptotic cell death of retinal ganglion cells in mouse glaucoma models. (A–G) Immunohistochemical analyses of retinas of mice administered PBS (control) or KUS121, 1 day after intravitreal injection of 5 nmol NMDA, interrogated with anti-CHOP (A, B), anti-NF-κB (C, D, E), and anti-single-strand DNA (ssDNA, F, G) antibodies. Arrowheads indicate CHOP- (A), nuclear translocated NF-κB- (D), and ssDNA-positive (F) retinal ganglion cells. (B, E, G) Quantification of CHOP- (B), nuclear translocated NF-κB- (E), and ssDNA-positive (G) retinal ganglion cells in NMDA-injected mice administered PBS (control, n = 3) or KUS121 (K121, n = 3). (H-L) Immunohistochemical analyses of 12-month-old GLAST (+/-) mice with anti-CHOP (H, I) and anti-NF-κB (J, K, L) antibodies. Arrowheads indicate CHOP- (H) and NF-κB-positive (K) retinal ganglion cells. (I, L) Quantification of CHOP- (I) and nuclear NF-κB-positive (L) cells in GLAST (+/-) mice administered PBS (control, n = 4 (NF-κB, CHOP)) or KUS121 (K121, n = 5 (NF-κB), n = 4 (CHOP)). *P < 0.05, **P < 0.001, ***P < 0.0001 (Aspin-Welch t-test). Scale bar: 50 μm.