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. 2016 Jul 14;16:477. doi: 10.1186/s12885-016-2461-4

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Experimental process for the two combined assays. a Schematic workflow for the identification of filtration-enriched CTCs by combined fluorescent staining and high-resolution cytomorphology. b Schematic workflow for detection of molecular biomarkers in filtration-enriched CTCs by combined fluorescent staining and fluorescent in situ hybridization (FISH)