Table 1.

FISH spots detected per CTC in a patient with an ALK-rearranged tumor depending on the number of z-stacks

CTC ID	Number of FISH spots
	5 z-stacks	10 z-stacks	15 z-stacks	20 z-stacks	25 z-stacks	30 z-stacks
1	n.ia	9	9	13	10	18
2	3	8	6	16	15	16
3	n.ia	3	2	8	7	12
4	4	11	2	4	13	13
5	n.ia	5	6	8	8	17
6	n.ia	12	4	4	2	16
7	8	6	8	19	1	27
8	2	4	4	8	10	15
9	2	2	3	13	7	16
10	n.ia	22	6	14	17	27
11	n.ia	4	8	8	10	16
12	1	10	6	16	12	16
13	1	4	2	16	2	3
14	n.ia	6	9	14	14	14
15	2	4	n.ia	4	4	16
16	1	10	8	2	7	16
17	n.ia	6	9	8	20	9
18	6	7	7	16	14	16
19	6	7	n.ia	10	12	18
20	3	8	6	6	8	18
21	1	3	9	6	8	8
22	2	5	6	n.i.a	14	8
%b	0 %	0 %	4 %	23 %	18 %	82 %

Abbreviations: ALK anaplastic lymphoma kinase gene; CTC circulating tumor cell; FISH fluorescence in situ hybridization; n.i. non-interpretable

^aNumber of FISH spots is uncountable due to non-optimal focus in the DAPI channel

^bPercentage of cases where a higher number of FISH spots were observed

The numbers in bold correspond to the highest number of spots for this CTC