Table 2.

FISH spots detected per CTC in a patient with a ROS1-rearranged tumor depending on the number of z-stacks

CTC ID	Number of FA-FISH spots
	5 stacks	10 stacks	15 stacks	20 stacks	25 stacks	30 stacks
1	9	n.ia	6	14	14	30
2	4	10	10	10	10	10
3	12	22	18	28	28	28
4	3	2	n.ia	13	14	14
5	4	4	4	4	4	4
6	26	28	26	34	34	34
7	6	15	11	20	28	28
8	36	42	48	52	62	62
9	10	14	18	20	30	30
10	13	20	22	26	30	30
11	2	12	12	13	14	14
12	6	6	8	10	14	14
13	4	6	6	8	14	14
14	8	13	20	32	32	32
15	10	14	14	16	16	16
16	10	12	13	16	16	16
17	2	6	4	15	16	16
18	4	11	16	25	25	25
19	20	33	26	46	55	64
20	12	14	12	14	15	14
21	11	n.ia	20	24	28	28
22	10	12	12	20	19	12
%b	4 %	9 %	9 %	41 %	86 %	91 %

Abbreviations: CTC circulating tumor cell; FISH fluorescence in situ hybridization; n.i. non-interpretable; ROS1 c-ros oncogene 1

^aNumber of FISH spots is uncountable due to non-optimal focus in the DAPI channel

^bPercentage of cases where a higher number of FISH spots were observed

The numbers in bold correspond to the highest number of spots for this CTC