Table 3.

FISH spots detected per CTC in a patient with an ERG-rearranged tumor depending on the number of z-stacks

CTC ID	Number of FA-FISH spots
	5 z-stacks	10 z-stacks	15 z-stacks	20 z-stacks	25 z-stacks	30 z-stacks
1	5	9	2	10	16	22
2	23	32	22	32	33	36
3	4	12	6	15	16	16
4	11	20	10	24	30	32
5	10	6	12	16	14	16
6	26	14	2	30	31	45
7	34	4	2	39	59	59
8	15	4	4	27	31	31
9	14	8	2	31	33	34
10	6	2	16	26	26	31
11	2	2	4	15	16	22
12	14	32	2	60	58	61
13	13	0	20	28	28	28
14	10	10	12	35	32	35
15	17	10	10	56	53	65
16	16	6	8	28	30	32
17	6	6	10	14	15	16
18	5	14	2	14	14	20
19	8	4	6	16	26	31
20	30	20	26	52	52	44
21	4	16	6	12	18	27
22	7	n.ia	6	30	29	30
%b	0 %	0 %	0 %	23 %	23 %	95 %

Abbreviations: CTC circulating tumor cell; ERG v-ets avian erythroblastosis virus E26 oncogene homolog; FISH fluorescence in situ hybridization; n.i. non-interpretable

^aNumber of FISH spots is uncountable due to non-optimal focus in the DAPI channel

^bPercentage of cases where a higher number of FISH spots were observed

The numbers in bold correspond to the highest number of spots for this CTC