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. 2016 Jul 15;9:143. doi: 10.1186/s13068-016-0559-1

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Total lignin content (%) of MYB32 and MYB42 transgenic sugarcane. Lignin content was quantified by a modified acid hydrolysis method. Acid-soluble lignin was determined by spectrophotometry and acid-insoluble lignin was measured gravimetrically. Acid-soluble lignin and acid-insoluble lignin were summed to determine total lignin content. a MYB31ORF, b MYB31UTR, c MYB42ORF and d MYB42UTR. Samples significantly different from the GFP controls after ANOVA followed by LSD test, p = 0.05, are shown with asterisk. Control n = 3. Data on all lines and statistical analysis are displayed in Additional file 4