Skip to main content
. 2016 Jun 10;35(14):1582–1595. doi: 10.15252/embj.201593561

Figure EV2. Expression levels and transcript lengths of wild‐type rsaA and its derivatives.

Figure EV2

  1. A schematic showing the location of RT–PCR products (green) examined for rsaA and the ruvA control. Transcripts are denoted as wavy red lines.
  2. RT–PCR products from RNA extracted from strains harboring the wild‐type rsaA or the derivative indicated. Samples where no reverse transcriptase was added were included to control for genomic DNA contamination.
  3. Western blot of FLAG‐tagged RsaA and its various derivatives indicated. Anti‐FLAG primary antibody and anti‐mouse secondary antibody were used.
  4. Contact probability plots showing interaction frequencies as a function of genomic distance for each strain examined by Hi‐C in Figs 2 and 3.
  5. Expanded view of the region surrounding the site of insertion (dashed line) for each strain examined in Fig 2.