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. 2016 Jun 3;35(14):1537–1549. doi: 10.15252/embj.201593489

Figure 1. DnaJC5 overexpression facilitates tau release into the extracellular space.

Figure 1

  1. Schematic illustrating the preparation of samples for media analysis by dot blot/Western blot or mass spectroscopy.
  2. Dot blot of tau levels in media from HEK293T cells overexpressing tau and FLAG‐DnaJC5 compared to empty vector and tau. Intracellular protein levels are shown by Western blot; DnaJC5 was detected by FLAG antibody. Quantification of extracellular tau levels is shown below; data are mean ± SEM, n = 8, ***P < 0.001.
  3. Light/heavy ratio of tau peptides in label‐free mass spectroscopy of media from HEK293T cells overexpressing tau or tau and DnaJC5 compared to 100 ng 15N‐labeled recombinant tau. Light peptides represent endogenous tau peptides. Data are mean ± SEM, n = 3 biological repeats, ***P < 0.001.

Source data are available online for this figure.