Fig. 6.

Effect of GMSYS on the phosphorylation of MAPKs and activation of NF-κB in LPS-stimulated RAW 264.7 macrophages. a The macrophages were pretreated with GMSYS (0, 250, 500, or 1000 μg/mL) for 2 h and then treated with LPS (1 μg/mL) for 15 min. Immunoblotting was used to detect the phosphorylation of p38 MAPK, ERK, and JNK in lysates prepared from the macrophages. b The macrophages were pretreated with GMSYS (0, 250, 500, or 1000 μg/mL) for 2 h and then treated with LPS (1 μg/mL) for 1 h. Immunoblotting of cytoplasmic and nuclear extracts prepared from the macrophages was used to detect the translocation of and subjected to immunoblotting for NF-κB p65. α-Tubulin and Lamin B were used as an internal control for cytoplasm and nucleus, respectively