. 2016 Jul 15;16:150. doi: 10.1186/s12866-016-0773-9

Table 1.

Growth and metabolic parameters of B. fragilis grown in glucose, EPS or without carbon source

Carbon source	Time (h)	Acetic acid	Propionic acid	Lactic acid	Succinic acid	Formic acid	Total metabolites*	Acetic/Propionic	Propionic/Succinic	Acetic/Lactic	Bacterial counts⁺
Glucose	24	13.00 ± 0.41^D	4.35 ± 0.63^C	14.81 ± 0.27^B	3.46 ± 0.19^{a B}	7.33 ± 0.15^D	42.95 ± 1.40^{a C}	3.02 ± 0.35^C	1.25 ± 0.12^A	0.88 ± 0.01^A	9.58 ± 0.07^{d C}
	48	13.28 ± 0.74^C	4.69 ± 0.21^C	17.11 ± 1.26^B	3.75 ± 0.27^{a B}	6.86 ± 0.38^D	45.73 ± 1.22^{ab D}	2.83 ± 0.08^D	1.25 ± 0.13^A	0.78 ± 0.07^A	8.47 ± 0.16^{c B}
	72	13.80 ± 0.53^C	5.04 ± 0.47^D	16.15 ± 1.01^B	4.37 ± 0.18^{b C}	7.59 ± 0.12^C	47.11 ± 0.41^{b C}	2.75 ± 0.18^D	1.15 ± 0.09^A	0.86 ± 0.09	7.76 ± 0.06^{b A}
	144	13.66 ± 0.26^D	4.96 ± 0.32^D	15.27 ± 0.87^B	4.56 ± 0.52^{b B}	7.26 ± 0.45^C	46.02 ± 1.96^{ab D}	2.76 ± 0.15^C	1.09 ± 0.06^A	0.90 ± 0.05^A	7.46 ± 0.17^{a B}
EPS E44	24	1.24 ± 0.06^{b B}	0.78 ± 0.08^{b A,B}	0.45 ± 0.07^{b A}	0.12 ± 0.02^A	0.43 ± 0.04^{a B}	3.01 ± 0.11^{a B}	1.60 ± 0.10^{a B}	6.78 ± 0.27^D	2.83 ± 0.53^{a A}	9.17 ± 0.12^{c B}
	48	0.93 ± 0.08^{a A,B}	0.39 ± 0.08^{a A}	0.95 ± 0.05^{c A}	-	1.27 ± 0.24^{b C}	3.56 ± 0.22^{b C}	2.44 ± 0.30^{b C}	ND	0.98 ± 0.12^{a A}	8.91 ± 0.02^{b C}
	72	1.89 ± 0.10^{c B}	0.95 ± 0.13^{b B}	0.77 ± 0.08^{c A}	-	1.67 ± 0.46^{b B}	5.31 ± 0.34^{c B}	2.01 ± 0.19^{ab C}	ND	2.48 ± 0.38^a	9.05 ± 0.05^{bc B}
	144	5.53 ± 0.08^{d C}	2.75 ± 0.13^{c C}	0.26 ± 0.15^{a A}	0.13 ± 0.05^A	1.40 ± 0.31^{b B}	10.07 ± 0.39^{d C}	2.01 ± 0.12^{ab B}	25.22 ± 11.55^B	26.24 ± 12.29^{b A}	8.68 ± 0.14^{a C}
EPS R1	24	1.70 ± 0.11^{a C}	1.37 ± 0.12^{b B}	0.16 ± 0.10^{b A}	0.26 ± 0.05^{b A}	0.73 ± 0.25^{a C}	4.22 ± 0.09^{a B}	1.25 ± 0.03^{a B}	5.40 ± 0.54^{b C}	14.03 ± 7.32^{a B}	9.01 ± 0.09^{b B}
	48	1.40 ± 0.22^{a B}	0.82 ± 0.12^{a B}	0.49 ± 0.09^{c A}	0.15 ± 0.01^{a A}	1.92 ± 0.12^{c B}	4.81 ± 0.37^{b B}	1.70 ± 0.08^{b B}	5.64 ± 0.55^{b B}	2.86 ± 0.12^{a B}	9.17 ± 0.09^{b D}
	72	2.08 ± 0.10^{b B}	1.44 ± 0.16^{b C}	0.12 ± 0.09^{b A}	0.26 ± 0.00^{b B}	1.35 ± 0.18^{b B}	5.25 ± 0.09^{b B}	1.45 ± 0.11^{a B}	5.62 ± 0.62^{b B}	25.26 ± 19.65^a	9.15 ± 0.13^{b B}
	144	3.13 ± 0.18^{c B}	1.70 ± 0.09^{c B}	0.02 ± 0.01^{a A}	0.53 ± 0.02^{c A}	1.27 ± 0.26^{b B}	6.65 ± 0.34^{c B}	1.85 ± 0.20^{b B}	3.21 ± 0.29^{a A}	>100^{b B}	6.73 ± 0.21^{a A}
WCS	24	0.21 ± 0.05^{a A}	0.26 ± 0.03^{a A}	-	0.11 ± 0.07^{a A}	0.09 ± 0.03^{a A}	0.72 ± 0.12^{a A}	0.79 ± 0.12^A	2.33 ± 0.43 ^B	ND	8.36 ± 0.15^{c A}
	48	0.27 ± 0.05^{a A}	0.34 ± 0.03^{b A}	-	0.21 ± 0.01^{b A}	0.17 ± 0.03^{b A}	1.01 ± 0.05^{a A}	0.79 ± 0.10^A	1.66 ± 0.21^A	ND	8.10 ± 0.06^{c A}
	72	0.35 ± 0.06^{a A}	0.36 ± 0.00^{b A}	-	0.20 ± 0.03^{b A}	0.17 ± 0.04^{b A}	1.08 ± 0.02^{a A}	0.99 ± 0.16^A	1.83 ± 0.29 ^A	ND	7.84 ± 0.11^{b A}
	144	0.66 ± 0.22^{b A}	0.51 ± 0.05^{c A}	-	0.23 ± 0.05^{b A}	0.26 ± 0.03^{c A}	1.68 ± 0.35^{b A}	1.28 ± 0.30^A	2.30 ± 0.36 ^A	ND	6.61 ± 0.19^{a A}

Metabolite concentrations are expressed in mM, bacterial counts are expressed in ufc mL⁻¹ Columns for the same carbon source with different lower case letter superscripts indicate significant differences along time (p > 0.05). Different capital letters superscripts indicate significant differences among carbon sources at the same sampling point (p < 0.05). * Sum of acetic, propionic, lactic, succinic, formic and pyruvic acids. ⁺ Counts at 0 h were 7.02 ± 0.08 log ufc mL⁻¹. WCS without carbon source. -, under the detection limit, ND Not determined