Fig. 4.

VGSC small RNAs persist until the adult stage of Ae. aegypti treated by dsRNA-VGSC during larval stage. a Small RNA libraries were constructed from 5 day-old adult mosquitoes previously soaked in VGSC₄₂₂ dsRNA or Random dsRNA (0.5 μg/μl) and sequenced using Hiseq 2500. Data are presented as the number of reads normalized to one million mapped reads per sample (RPM). Forward strand (sense) reads are positive RPM values (depicted in blue); reverse strand (anti-sense) reads are in negative RPM values (depicted in red). Positions for which RPM values are > = 10 RPM were considered expressed. b Size distribution of small RNAs matching the VGSC422 transcript (Accession no. KC107440.1). nt: nucleotides