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. 2016 May 12;2(5):e00110. doi: 10.1016/j.heliyon.2016.e00110

Fig. 6.

Fig. 6

SDS–PAGE of purified NtPGIP recombinant protein via Ni-Sepharose affinity chromatography. Lane 1: product eluted with 20 mmol/L imidazole; Lane 2: product eluted with 100 mmol/L imidazole; Lane 3: product eluted with 200 mmol/L imidazole; and Lane 4: product eluted with 400 mmol/L imidazole. S: supernatants; P: precipitation; N: total protein from strains harboring pET-PGIP after induction for 4 h under 37 °C; M: Protein molecular weight marker. A full, unmodified version of this figure is available as Supplementary File 1.