Fig. 5.

Effect of Ca-deficient diet on CCl₄-induced liver inflammation and CYP induction. Mice were fed a normal diet or a Ca-deficient diet from 4 weeks of age for a period of 4 weeks. At 8 weeks of age, the mice were injected intraperitoneally with 2 g/kg CCl₄. Twenty four hours after CCl₄ injection, total RNA and total protein were isolated from the mice livers. Quantitative RT-PCR analysis and ELISA were performed. The amount of quantified target mRNA was normalized against GAPDH mRNA. (A), (C), and (D) indicate TNFα, IL-6, and CYP2E1, respectively. (B) indicates hepatic TNF-α level. Data indicate mean ± S.D. for 6–9 mice. ^**P < 0.01 versus control, ^#P < 0.05 and ^##P < 0.01 versus normal diet + CCl₄ group. N.S.: not significant.