Fig. 5.

Glucose stimulated insulin secretion from pseudo-islets and intact islets. (A–D) Pseudo-islets formed in 100 μm, 300 μm, and 500 μm microwells (n = 6) and cultured intact islets (control; n = 6) were incubated with 3.3 mmol/L glucose and subsequently 20 mmol/L glucose at (A, C) day 7 and (B, D) day 14. Insulin concentration was normalized to (A, B) the DNA content in the islet cells or (C, D) the absorbance values from WST-8 assay. Data are presented as the mean ± SD. *Significant differences between groups (p < 0.05).