Fig. 5.

CYR61 regulation of ERK and AKT phosphorylation and dexamethasone-inhibited cell migration. HCT116 cells were treated for 48 hours with transforming growth factor β1 (TGF-β1, 10 ng/mL), or with dexamethasone (10 μM) combined with hrCYR61 (300 ng/mL) (A) or AbCYR61 (500 ng/mL) (B). ERK and AKT phosphorylation were detected by western blot analysis with the indicated antibodies. (C) CYR61 mRNA expression levels were examined by reverse-transcriptase polymerase chain reaction using primers designed against the indicated targets. (D) HCT116 cells were treated with dexamethasone (1, 2, or 5 μM) alone, or co-treated with TGF-β1 (10 ng/mL) and/or the ERK inhibitor PD98059 (50 μM) or the AKT inhibitor LY49002 (50 μM). CYR61 protein expression was examined by western blot analysis with the indicated antibodies. Data are represented as the mean percent migration distance±standard deviation of at least three replicates. ^*p < 0.05 in all experiments. GAPDH, glyceraldehyde 3-phosphate dehydrogenase.