Fig. 3.

The percentage of sub-diploids cells by flow cytometry analysis after treatment with 0 to 160 μg/mL of α-zingiberene on SiHa cells for 24 hr. Cells were stained with PI, and the number of sub-diploids cells were countered using FACScan flow cytometry. Cells with a subdiploid DNA content (> 5% of G₀ content) were considered to be apoptotic. The distribution of cell cycle was analysed with ModiFitLT V 2.0. Data point represents mean values of five replicates, with bars indicating s.e.m. *p < 0.05, **p< 0.01 compared to control.