FIG 5.

Normal mitochondrial abundance and energy-sensing signaling in tissues from Mpc1 KI hypomorph embryos. (A) Representative immunoblot for phosphorylation (p-AMPK and p-mTOR) of canonical energy-sensing components of AMPK and mTOR signaling pathways. (B) There was no difference in mitochondrial DNA content as determined by qRT-PCR with primers specific to the mitochondrial Nd1 gene relative to nuclear loci on Chr7 and Chr12 (means plus SEM; n = 7 or 8). (C) Representative immunoblot for mitochondrial proteins in WT and Mpc1^KI/KI cerebral cortex, heart, and liver, with Hsc70 as a loading control. CV, complex V; CIII, complex III; CII, complex II; CI, complex I.